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KMID : 0382420030290020023
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Korean Journal of Environmental Health Society
2003 Volume.29 No. 2 p.23 ~ p.28
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Effect of Cyclohexane Treatment on the Liver Cytochrome P-450 Dependent Aniline Hydroxylase Activity in Alcohol-pretreated Rats
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Kim Byung-Ryul
Yoon Chong-Guk
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Abstract
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To evaluate the effect of cyclohexane(CH) treatment on the liver cytochrome P-450 dependent aniline hydroxylase(CYPdAH) activity in alcohol-pretreated animals, CH( 1.56 g/kg body weight) was intraperitoneally administered to Sprague-Dawley male mts, which had been drunk 15% alcohol in distilled water for 1,3 and 6 weeks. CH was injected to rats 4 times every other day and the animals were sacrificed at 24 hours after injection of CH. In the alcohol-pretreated mts, liver injuries were not demonstrated on the basis of the liver weight per body weight, the levels of serum alanine aminotransferase and hepatic microsomal glucose-6-phosphatase activities. By the CH treatment, alcohol-pretreated animals showed the significantly increased activity of hepatic microsomal CYPdAH. Concomitantly V max value in CYPdAH was more increased. whereas KM value more decreased in alcohol-pretreated animals by the treatment of CH. In conclusion. the increasing cause of microsomal CYPdAH in CH-treated mts pretreated with alcohol may be due to induction of enzyme protein in rat liver.
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KEYWORD
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